%example for psfgen.m % function is called with pairs of arguments: % first is the name, second the value: % lambda - wavelength of emission light [nm] % na - numerical aperture % pixelisze - size of the image pixel [nm] % sizevec - vector with number of pixels in X and Y direction % method - 'airy' - airy disk (scalar approximation) % - 'gauss' - gaussian approximation [Zhang et al., 2007] % verbose - print out the parameters % eg: psf_odd = psfgen('lambda', 600, 'na', 1.4, 'pixelsize', 110, 'sizevec', [11 11], 'method', 'airy'); imagesc(psf_odd) colormap(gray) set (gca, 'DataAspectRatio', [1 1 1] ); title('psf - odd sizevec') % generates 11x11 pixels image os PSF for 600nm emsion wavelength, 1.4NA objective with 110nm % pixels-size using airy approximation of PSF (airy disk) % for odd number of pixelsize PSF is placed in center. For even pixelsize % PSF is centered on the upper-left pixel next to center... psf_ev = psfgen('lambda', 600, 'na', 1.4, 'pixelsize', 110, 'sizevec', [10 10], 'method', 'airy'); figure imagesc(psf_ev) colormap(gray) set (gca, 'DataAspectRatio', [1 1 1] ); title('psf - even sizevec') %calling without some (or any) arguments generates pfs default values of %missing arguments: % eg: psf_def = psfgen('lambda', 800, 'method', 'gauss'); figure imagesc(psf_def) colormap(gray) set (gca, 'DataAspectRatio', [1 1 1] ); title('psf - default values') % generates PSF with for 800nm emission wavelength, using Gaussian % approximation of the PSF and the rest of arguments will be set to default % values: % default values: % na = 1.2; % pixelsize = 100; %nm % sizevec = [25 25]; % All default values: % lambda = 520; %nm % na = 1.2; % pixelsize = 100; %nm % method = 'airy'; % sizevec = [25 25]; % or type help psfgen